Figure 1. Overview of integrative structural network biology of native cell extracts in a thermophilic eukaryote.

• We combined computational modeling approaches adapted from network biology (molecular profiling) with molecular biophysics, electron microscopy (EM; structural profiling), and quantitative and cross‐linking mass spectrometry (interface profiling) to systematically chart and characterize the organization of protein complexes into functional, local communities. Large‐scale electron microscopy and cross‐linking mass spectrometry are used as validation tools.