Table 1.
Antibody ID | Target residues | EC‐50 (nM) | Relative amount (%) a | Peptide 5.1 (aa 236–363) (%) b | Peptide 5.2 (aa 347–492) (%) b | Peptide 5.3 (aa 364–530) (%) b |
---|---|---|---|---|---|---|
Ab‐5 | 274–400 | 1.9 | 100 | 66 | 29 | 24 |
Ab‐N | 274–346 | 4.5 | 18 | 86 | 1 | 1 |
Ab‐M | 347–363 | 0.7 | 35 | 20 | 18 | 1 |
Ab‐C | 364–400 | 1.2 | 39 | 1 | 94 | 87 |
Ab‐S | 274–400 | N/A | 8 | 1 | 2 | 2 |
Polyclonal antibodies raised against Immunogen 5 were analyzed in terms of composition, (relative amounts after fractionation) apparent affinities (EC‐50) and specificity (reactivity towards peptide 5.1–5.3).
Numbers are percentages of respective antibody eluted, relative the total amount of Immunogen 5 specific antibodies.
Numbers are percentages of binding intensities values for respective antibody relative binding to Immunogen 5.