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. 2009 Jan 31;3(3):238–247. doi: 10.1016/j.molonc.2009.01.003

Table 1.

‐ Characteristics of fractionated antibodies.

Antibody ID Target residues EC‐50 (nM) Relative amount (%) a Peptide 5.1 (aa 236–363) (%) b Peptide 5.2 (aa 347–492) (%) b Peptide 5.3 (aa 364–530) (%) b
Ab‐5 274–400 1.9 100 66 29 24
Ab‐N 274–346 4.5 18 86 1 1
Ab‐M 347–363 0.7 35 20 18 1
Ab‐C 364–400 1.2 39 1 94 87
Ab‐S 274–400 N/A 8 1 2 2

Polyclonal antibodies raised against Immunogen 5 were analyzed in terms of composition, (relative amounts after fractionation) apparent affinities (EC‐50) and specificity (reactivity towards peptide 5.1–5.3).

a

Numbers are percentages of respective antibody eluted, relative the total amount of Immunogen 5 specific antibodies.

b

Numbers are percentages of binding intensities values for respective antibody relative binding to Immunogen 5.