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. 2008 Nov 6;3(1):54–66. doi: 10.1016/j.molonc.2008.10.005

Figure 5.

Figure 5

(A) Transcriptional repression of β‐catenin signaling by PCDH24. To perform the reporter assay, we first analyzed PCDH24 expression in SW480 by Western blotting. PCDH24 was not detected, as in other colon cancer cell lines. The name of each cell line is indicated at the top of the panel and the arrow indicates the MW of the PCDH24 protein. These results are in accordance with our previous results by RT‐PCR (Okazaki et al., 2002). (B) Downregulation of TCF/lef signaling by wild‐type and mutant (L1310R) PCDH24 expression in SW480 cells. The ratio of luciferase activities in cells transfected with TOPFLASH versus FOPFLASH reporters was calculated. Means and standard deviations of quadruplicate measures are shown. The amount of PCDH24 expression plasmids was changed from 0.2 to 1.7μg and that is indicated at the bottom of the panel.