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. 2008 Nov 1;3(1):67–76. doi: 10.1016/j.molonc.2008.10.002

Figure 1.

Figure 1

HBV genome expression in HepG2.2.15. A. ELISA assay detection of HBsAg secreted in cell culture medium of both HepG2 and HepG2.2.15. Data are presented as an S/CO ratio (Y‐axis), which stands for the Sample Rate/Cut‐Off Rate, calculated by the IMx system. S/CO ratios >1 are considered to be reactive while ratios <1 are non‐reactive. The positive control and negative control are reagents provided in the IMx kit. B. RT‐PCR performed to show the HBsAg and HBcAg expression in the mRNA of both the cell lines. The negative control was no template DNA added but equal volume of water instead.