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. 2010 Feb 2;4(2):135–149. doi: 10.1016/j.molonc.2010.01.004

Figure 5.

Figure 5

Effect of rHuEPO on the transcriptional effect of Testosterone–BSA on EPOR. A. Effect of rHuEPO, Testosterone–BSA (10−7M) or their equimolar association on luciferase activity in lysates from T47D cells transiently transfected with the short or the long promoter construct, after 24h incubation. *p<0.05 at least, mean±SEM of three assay. B. Quantitative RT‐PCR of EPOR in T47D cells incubated with either agent (10−7M) or their equimolar association, for the indicated time periods. *p<0.05 at least compared to control (non treated cells). +p<0.05 at least compared to testosterone–BSA or rHuEPO‐treated cells, #p<0.05 compared to testosterone–BSA‐treated cells; Mean±SEM of three assays. C. Effect of kinase inhibitors SB203580 (p38 MAPK inhibitor), Wortmannin (pI3K inhibitor), AG490 (JAK2 inhibitor) and SP600125 (JNK inhibitor) on T47D cells, transiently transfected with the short (left panel) or the long (right panel) EPOR promoter transcript and incubated with Testosterone–BSA, or an equimolar association of rHuEPO and Testosterone–BSA (10−7M).