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Validation of microarray gene expression by real‐time PCR. A–D: Relative expression levels of genes identified by SAM; FOXA1, TRAF3IP3, HCST and CYP1B1, measured by TaqMan qRT‐PCR in 22 type II and 6 type I DCIS cases. E–G: Relative expression level of EMT‐related genes identified by SAM; SNAI1, S100A8 and CXCL1, measured by TaqMan qRT‐PCR in 22 DCIS type II and 6 DCIS type I cases. Black horizontal bars represent median value for each DCIS phenotype.
