Table Table 3.
Validation of microarray data by quantitative PCR. Total RNA samples were subjected to qPCR as described in detail in Section 5. A: For quantification pooled RNA (four pools with n=5, representing irradiated and non‐irradiated tumour samples) or single RNA samples (n=10) were used, as indicated. Values are depicted as fold‐change±SD (microarray) and relative expression±SD (qPCR). B: The relative expression of four genes from RNA of single tumour samples representing different TMN stages was determined. Values are depicted as relative expression±SD.
| A | ||||
|---|---|---|---|---|
| Gene symbol | Fold‐change | Relative expression | ||
| (microarray) | (qPCR) | |||
| IGF1a | ↑ 2.73±0.69 | ↑ 14.22±4.36 | ||
| ANGPT1a | ↑ 2.38±0.59 | ↑ 9.60±4.99 | ||
| PTGER3a | ↑ 2.39±0.19 | ↑ 25.43±17.80 | ||
| DPTa | ↑ 4.26±1.02 | ↑ 103.84±19.60 | ||
| MYBa | ↓ −3.17±0.32 | ↓ 0.11±0.08 | ||
| FABP6a | ↓ −3.26±0.84 | ↓ 0.02±0.05 | ||
| COL6A1 | ↑ 2.22±0.47 | ↑ 13.91±15.99 | ||
| MMP2 | ↑ 2.56±0.58 | ↑ 23.16±11.38 | ||
| CEACAM1 | ↓ −3.06±0.02 | ↓ 0.08±0.05 | ||
| PCDH1 | ↓ −1.09±0.45 | ↓ 0.67±0.24 | ||
| B | ||||
| Gene symbol | TNM Stage I (2) | TNM Stage II (2) | TNM Stage III (4) | TNM Stage IV (2) |
| COL6A1 | ↑ 10.14±7.05 | ↑ 18.28±11.30 | ↓ 0.77±0.76 | ↑ 2275.55±4.20 |
| MMP2 | ↑ 13.56±1.28 | ↑ 27.70±0.99 | ↑ 10.35±6.14 | ↑ 78.90±11.13 |
| CEACAM1 | ↓ 0.06±0.01 | ↓ 0.93±0.37 | ↓ 0.04±0.01 | ↓ 0.02±0.001 |
| PCDH1 | ↑ 1.18±0.05 | ↑ 2.72±0.18 | ↓ 0.17±0.04 | ↓ 0.65±0.28 |
↑, up‐regulated, ↓, down‐regulated; SD, standard deviation; number of patients.
pooled samples.