Fig 3.

TGX221 analogs inhibits AKT S473 phosphorylation. A–D. Prostate cancer cells (PC-3, DU145, 22RV1 and C4-2) were serum-starved overnight and then pre-treated with the solvent DMSO or the compounds as indicated at 5 μM for 30 min. Cells were then stimulated with FBS (10%) for 30 min. Cells were harvested and Western blot was done with anti-AKT (total and pS473) antibodies. Actin blot served as protein loading control. E. The relative band density of pAkt S473 after normalized against total Akt levels were summarized in panel E. Data are representative of three separate experiments.