Figure 4. Bacterial colony growth on LB plates containing carbenicillin to select for circular pSPL3 plasmid-carrying bacteria.

JM109 bacterial cells were transformed with either ‘insert + vector’ (panels A-C) or ‘vector only’ (panels D-F) ligation products and 100 μl was plated undiluted (panels A and D), at a 1:10 dilution (panels B and E), or at a 1:100 dilution (panels C and F).