| gDNA template (20 ng/μl) | 3 μl |
| 5x Phusion HF buffer | 4 μl |
| 2 mM dNTPs | 2 μl |
| 10 μM forwards primer | 1 μl |
| 10 μM reverse primer | 1 μl |
| Phusion polymerase (2 U/μl) | 0.2 μl |
| Nuclease-free water | 8.8 μl |
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