| Initial denaturation | 98 °C | 30 sec |
| 35 cycles of | 98 °C | 10 sec (denaturation) |
| 50-72 °C | 30 sec (annealing temperature of primers) | |
| 72 °C | 30 sec per kb amplicon (extension) | |
| Final extension | 72 °C | 10 min |
| Hold temperature | 4 °C |
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