| Vector sample | Insert sample | |
| DNA | 4 μg | 2 μg |
| BamHI enzyme (20 U/μl) | 4 μl | 2 μl |
| XhoI enzyme (20 U/μl) | 4 μl | 2 μl |
| 10x NEBuffer 3.1 | 20 μl | 10 μl |
| Nuclease-free water | Make up to 200 μl | Make up to 100 μl |
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