10x RT buffer | 2 μl |
25x dNTPs (100 mM) | 0.8 μl |
10x RT random primers | 2 μl |
RNase inhibitor at 1 U/μl | 1 μl |
Nuclease-free H2O | 3.2 μl |
MultiScribe reverse transcriptase | 1 μl |
10x RT buffer | 2 μl |
25x dNTPs (100 mM) | 0.8 μl |
10x RT random primers | 2 μl |
RNase inhibitor at 1 U/μl | 1 μl |
Nuclease-free H2O | 3.2 μl |
MultiScribe reverse transcriptase | 1 μl |