Table A4.
Numbers of sequences per assay, per site; “Unfiltered” refers to sequences that have been 100% matched to the sequence specific primers, the MID tags, and the adaptor sequence
| Site | Sequence type | Ceph 16S | S_Ceph 16S | Fish 16S | Plank COI | Crust 16S |
|---|---|---|---|---|---|---|
| Houtman Abrolhos | Unfiltered | 36225 | 114540 | 56420 | 30530 | 34167 |
| Mean unique | 395 ± 431 | 694 ± 453 | 1487 ± 367 | 324 ± 138 | 622 ± 504 | |
| Filtered and assigned | 33164 | 108929 | 42584 | 28499 | 30999 | |
| Mean unique | 55 ± 16 | 95 ± 13 | 34 ± 25 | 65 ± 25 | 34 ± 12 | |
| Beagle Islands | Unfiltered | 23183 | 147703 | 73954 | 42126 | 30913 |
| Mean unique | 327 ± 403 | 555 ± 208 | 1685 ± 479 | 309 ± 126 | 898 ± 518 | |
| Filtered and assigned | 19909 | 140073 | 53570 | 38603 | 25559 | |
| Mean unique | 35 ± 14 | 88 ± 23 | 25 ± 4 | 77 ± 32 | 47 ± 21 | |
| Shoalwater Bay | Unfiltered | 34613 | 146541 | 92175 | 52095 | 81945 |
| Mean unique | 331 ± 250 | 479 ± 331 | 1385 ± 745 | 259 ± 148 | 862 ± 266 | |
| Filtered and assigned | 29110 | 125647 | 50835 | 41130 | 41899 | |
| Mean unique | 46 ± 19 | 81 ± 22 | 26 ± 18 | 43 ± 18 | 51 ± 8 | |
| Fitzgerald River | Unfiltered | 7754 | 50363 | 94898 | 15122 | 0 |
| Mean unique | 168 ± 125 | 368 ± 143 | 2301 ± 742 | 198 ± 113 | 0 | |
| Filtered and assigned | 3624 | 45758 | 57549 | 14045 | 0 | |
| Mean unique | 59 ± 47 | 74 ± 11 | 37 ± 25 | 60 ± 40 | 0 | |
| Recherche Archipelago | Unfiltered | 332 | 55926 | 36321 | 20501 | 0 |
| Mean unique | 50 | 534 ± 124 | 1239 ± 165 | 218 ± 160 | 0 | |
| Filtered and assigned | 325 | 46432 | 27500 | 19472 | 0 | |
| Mean unique | 48 | 62 ± 31 | 29 ± 13 | 62 ± 42 | 0 |
“Filtered and assigned” refers to the number of sequences that have passed through the Usearch filtering process and were assigned to taxa. “Mean unique” refers to the mean number of unique sequences produced given the number of positive samples for the assay.