Table 3. Primers used to amplify turkey mtDNA, their coordinates relative to the turkey (EF153719; [30]) mtDNA reference sequence, amplicon lengths produced, and annealing temperatures.

While primers were previously reported by Speller et al. [22], the coordinates of T15533F are corrected here, the target regions have been renamed, and the amplicon sizes are reported.

Target Region	Primer	Coordinates	Sequence (5' to 3')	Amplicon Length (bps)	Annealing Temperature
A1	T15533F	15533–15553	GTTGTTCTCAACTACGGGAAC	124	55°C
	T15656R	15634–15656	GTATGTGGTATATAAATGTATCG
A2	T15612F	15612–15633	GGGGTATACTATGCATAATCGT	139	55°C
	T15750R	15730–15750	GTAGTCATAGGGAGAAATGG
B	T15709F	15709–15729	ACGGACATAACAACCTTTACC	186	60°C
	T15894R	15875–15894	TCTGGTACGTCGAGCATAAC
C	T15853F	15853–15874	CTTACTGTACTTACCCCATTTG	180	60°C
	T16032R	16014–16032	TCGACCGAGGAACCAGAGG