Table 4. Primers used to amplify dog and coyote mtDNA, their coordinates relative to the dog (U96639; [34]) and coyote (DQ480509; [35]) mtDNA reference sequences, amplicon lengths produced, and annealing temperatures.

Target Region	Primer	Coordinates to Dog mtDNA	Coordinates to Coyote mtDNA	Sequence (5' to 3')	Dog Amplicon Length	Coyote Amplicon Length	Annealing Temperature	Reference
DOG 1	D15401F	15401–15420	15403–15422	AAGCTCTTGCTCCACCATCA	195	191	60°C	This study
	D15595R	15571–15595	15569–15593	GATATAATATTATGTACATGCTTAT
DOG A	D15421F	15421–15440	15423–15442	GCACCCAAAGCTGAGATTCT	197	193	58°C	Witt et al. [11]
	D15617R	15995–15617	15993–15615	GAGTTAATATGTCCTATGTAAGG
DOG 2	D15534F	15534–15555	15532–15553	CTATGTACGTCGTGCATTAATG	178	178	60°C	This study
	D15711R	15692–15711	15690–15709	GGTTGATGGTTTCTCGAGGC
DOG B	D15595F	15595–15617	15593–15615	CCTTACATAGGACATATTAACTC	202	202	60°C	Witt et al. [11]
	D15796R	15776–15796	15774–15794	AGAACCAGATGCCAGGTATAG
COYOTE 1	C15401F	15399–15416	15401–15418	AGAAGCTCTTGCTCCACC	199	195	55°C	This study
	C15595R	15575–15597	15573–15595	AAGATATAATATTATGTACATGC
COYOTE 2	C15551F	15553–15570	15551–15568	ATGGCTTGCCCCATGCAT	180	180	55°C	This study
	C15730R	15714–15732	15712–15730	AAGAGGGACATTACGAGCA