Table 4.
Validation by pyrosequencing: genes (15) that were differentially methylated and contributed significantly to the formation of methylation clusters assessed by Golden Gate array were reanalyzed in a larger dataset by pyrosequencing. The association to mRNA expression derived classes and grade was confirmed. Kruskal–Wallis H test was used to determine the statistical significance.
| Subtypes (mRNA expression) | Histological grade | |
|---|---|---|
| p‐value | ||
| BMP3 | 0.000 | 0.002 |
| GUCY2D | 0.000 | 0.001 |
| GUCY2F | 0.000 | 0.148 |
| HDAC1 | 0.000 | 0.000 |
| OGG1 | 0.000 | 0.000 |
| TFF1 | 0.000 | 0.000 |
| ASCL2 | 0.326 | 0.344 |
| HOXA11 | 0.000 | 0.000 |
| IGFBP7 | 0.000 | 0.003 |
| LYN | 0.000 | 0.000 |
| ROR2 | 0.009 | 0.102 |
| HCK | 0.101 | 0.000 |
| BCAP31 | 0.000 | 0.000 |
| FZD9 | 0.000 | 0.001 |
| ST6GAL1 | 0.000 | 0.000 |