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. 2011 Oct 20;5(6):517–526. doi: 10.1016/j.molonc.2011.10.004

Figure 2.

Figure 2

FAK inhibitors FI14 and PF‐228 inhibit FAK kinase activity in vitro. FAK was immunoprecipitated from HUVEC total protein lysates and in vitro kinase assays were performed as described in Section 2.4, in the presence or absence of exogenously added recombinant paxillin as a kinase target substrate. Membranes were exposed to film to reveal the autoradiographic signals for phospho‐FAK and phospho‐paxillin (indicated by autorad panels). Membranes were subsequently analyzed by western blot for total FAK and paxillin as a loading control (indicated by IB panels). The amount of FAK autophosphorylation and paxillin target substrate phosphorylation by FAK was reduced by the presence of FAK inhibitors compared to vehicle control.