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. 2011 Oct 20;5(6):517–526. doi: 10.1016/j.molonc.2011.10.004

Figure 5.

Figure 5

FAK inhibitors block HUVEC sprouting on collagen I gels. For assessing formation of sprouts, HUVEC were seeded onto collagen I gel‐coated plates and treated with 50 ng/ml VEGF in the absence (with added DMSO as a vehicle control) or presence of increasing concentrations of FAK inhibitors. HUVEC sprouting assay as a function of time (A). Sprouts were counted every 2 days in 10 random fields of view at 100× magnification. Mean number of sprouts per high powered field (HPF) and its associated standard error from duplicate dishes in each of two independent experiments are shown over time. Asterisks denote statistically significant differences (***p < 0.001) as determined by one‐way ANOVA analysis between cells treated with each of the drugs and vehicle. Sample images from day 8 of a representative experiment are shown (B). The effect of FAK inhibitors on sprout formation on collagen I was dose‐dependent.