Figure 2.
Binding of targeted toxins and EGF to HER14 cells. Binding activity was determined in a cell based ELISA. All targeted toxins and EGF bound to the cells. Sap‐EGF, Dia‐EGF, RTA‐EGF and DT390EGF were detected with an anti‐His‐, EGF‐ETA' with an anti‐PE‐ and EGF with an anti‐EGF antibody. Each column represents the mean ± SD of one experiment performed in quadruplicate, n = 4. Values (expressed as quotient of absorption) were normalized to control cells that were only treated with antibodies. ∗Significant to control (anti‐His, anti‐PE, anti‐EGF), Mann‐Whitney‐Test, p ≤ 0.04.