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Binding of SA1641 to Hissaporin. Binding of SA1641 to Hissaporin was determined by surface plasmon resonance (SPR). Hissaporin was immobilized on a sensor chip with a shift of approximately 770 resonance units (RU) and SA1641 was injected at following concentrations: 5, 10 or 20, μg/mL with PBS (pH 7.4) or sodium acetate (pH 5) running buffer. Each concentration was applied in duplicate. At pH 5 the binding of SA1641 to Hissaporin was increased.
