Figure 6.

Doxorubicin and cisplatin induce phosphorylation of HER3 while other chemotherapeutic drugs do not. (A) Cell lines were treated with indicated concentrations of different chemotherapeutic drugs for 24 h. Phosphorylation of HER3 was analysed with the P‐HER3 specific antibody (P‐tyr 1289). Tubulin serves as loading control. (B) Nine different ovarian cancer cell lines are grouped based on chemotherapeutic pre‐treatment of cancer patients from where cell lines have originally been established (Dox = doxorubicin; Cis = cisplatin; Cyclo = cyclophosphamide; Carbo = carboplatin). The doxorubicin‐induced increase in HER3 and AKT phosphorylation is indicated. (C) Hypothetical cell signalling model representing the pathway which is induced upon addition of doxorubicin.