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. 2012 Oct 23;7(1):121–129. doi: 10.1016/j.molonc.2012.10.001

Figure 2.

Figure 2

Both Gi and G13 are required for EGFR phosphorylation. (A) The serum‐starved cells were pretreated with PTX for 1 h and then stimulated with EGF or LPA for 5 min. EGFR phosphorylation by immunoprecipitation and immunoblotting. (B) The cells were transfected with indicated siRNA and then stimulated with or without LPA for 5 min. EGFR phosphorylation by immunoprecipitation and immunoblotting. Densitometric analysis is representative of three independent experiments. The histograms show the ratio between active and total EGFR protein levels (Error bars, ± s.d. *p < 0.05 vs control, $p < 0.05 vs LPA treatment only). (C) The cells were transfected with indicated siRNA. G13 and G12 expression by immunoblotting (Error bar, ± s.d. **p < 0.01 vs negative control siRNA only).