Figure 2.

Vorinostat induces apoptotic cell death on endometrial cancer cell lines. A, Quantification of Hoechst‐stained apoptotic nuclei of IK, HEC and RL cells treated for 24 h with increasing doses of Vorinostat. Results are expressed as percentage of viability over control values. B, IK cells were treated with Vorinostat 5 μM Vorinostat for the indicated periods of time or left untreated (UN). Cell lysates were prepared, and 20 μg protein was electrophoresed, blotted, and probed with the indicated antibodies. C, HEC‐1‐A and D, RL‐95 cells were treated with increasing doses of Vorinostat for 24 h. Cell lysates were prepared and 20 μg of protein were electrophoresed, blotted, and probed with the indicated antibodies. Anti‐tubulin was used to ensure equal protein loading and anti‐acetylated‐H4 was used to ensure that Vorinostat treatment was effective.