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. 2013 Mar 28;7(4):763–775. doi: 10.1016/j.molonc.2013.03.003

Figure 3.

Figure 3

Downregulation of FLIP is not involved in anti‐tumoral activity of Vorinostat. A, IK, HEC‐1A and RL‐95 cells were treated with increasing doses of Vorinostat for 24 h. Cell lysates were subjected to Western Blot with Acetylate H4, anti‐FLIP antibody and tubulin antibody. B, IK cells were treated with indicated doses of Vorinostat and cell lysates were analyzed by quantitative RT‐PCR to determine the levels of FLIP mRNA. C, IK cells transfected with either control (pEIGW) or a plasmid expressing FLIP (pEIGW‐FLIP) and treated with 3 μM Vorinostat and after indicated times protein cell lysates were subjected to Western Blot with antibodies against Caspase‐3, anti‐Flag to ensure specific overexpression of FLIP and anti‐tubulin to ensure equal protein loading. D, Representative images and quantification of clonogenic ability of IK, HEC‐1‐A and AN3CA cells infected with lentiviruses carrying either control (pEIGW) or overexpression of FLIP (pEIGW‐FLIP) plasmid and left untreated (UN) or treated with 3 μM Vorinostat (VOR).