Figure 5.

Hsp90 inhibitors can overcome crizotinib resistance caused by EMT. (A) Cells were treated with the indicated doses of 17‐DMAG or AUY‐2922. Cell viability was measured 72 h later using the MTT assay. Bars represent the standard deviations. (B) Cells were treated with or without the indicated doses of 17‐DMAG or AUY‐922 for 3 h. Changes in ALK‐related signaling and TGF‐β receptors were analyzed using Western blot analysis. The densitometry values were determined relative to control of H2228 after normalization to β‐actin protein. (C) Cells were treated with crizotinib (1 μM), 17‐DMAG (1 μM), or AUY‐922 (1 μM) for the indicated times. Cell lysates were subjected to Western blot analysis using the indicated antibodies. The densitometry values were determined relative to control after normalization to β‐actin protein.