Skip to main content
. 2013 Sep 3;7(6):1142–1151. doi: 10.1016/j.molonc.2013.08.009

Figure 3.

Figure 3

Ligation with RG7287 antibody translocates CDCP1 to detergent insoluble fraction. H322M cells were incubated with either 10 μg/ml of control IgG or (A) RG7287 antibody or (B) the Fab fragment of RG7287 for the indicated times. After lysis in 1% Triton X‐100 buffer, the insoluble fraction was prepared and analyzed by Western blotting. The insoluble fraction was isolated and analyzed by Western blotting with antibodies against phospho‐ and total CDCP1, phospho‐ and total Src, the lipid raft marker protein flotillin.