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. 2013 Aug 16;7(6):1043–1055. doi: 10.1016/j.molonc.2013.08.003

Figure 4.

Figure 4

Ad‐TRAIL‐3MREs infection induced apoptosis in uveal melanoma cells. (A) Cleaved fragments of caspase 3, 8 and 9, and PARP, as well as truncated Bid, was detected by immunoblotting assays, in order to confirm the activation of apoptotic pathway. GAPDH was selected as endogenous controls. (B) Apoptotic rates were determined in ARPE‐19, L‐02, SP6.5 and OM431 cells 48 h after infection with Ad‐TRAIL, Ad‐TRAIL‐3MREs or Ad‐EGFP of 10 MOI by FACS‐based detection of Annexin V positivity. Means ± SD of three separate experiments were represented. (C) Apoptosis was detected in L‐02 and SP6.5 cells with Hoechst 33342 staining (×200). Arrows indicated the apoptotic cells with nuclear condensation.