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. 2013 Aug 19;7(6):1056–1068. doi: 10.1016/j.molonc.2013.08.005

Figure 1.

Figure 1

Evaluation of a specific binding domain (BD) for efficient trans‐splicing. (A) Schematic diagram of the screening procedure used for evaluation of BDs. (B) Binding position of RTM_1 within the target intron 3 of SLCO1B3 (nt1012‐nt1118). (C) Analysis of AcGFP expression by flow cytometry in HEK293AD cells transfected with the target molecule, RTM_1, or both the target molecule + RTM_1. Only cells co‐transfected with the target molecule and RTM_1 showed green fluorescence signal, resulting from fusion of both parts of the split AcGFP gene by RNA trans‐splicing. BD: Binding Domain, BP: Branch Point, PPT: Poly Pyrimidine Tract, 3′ ss: 3′ splice site.