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. 2012 Dec 12;7(3):419–427. doi: 10.1016/j.molonc.2012.11.005

Figure 1.

Figure 1

The E1A splice variant of p150 under control of the P2 SALL2 promoter is expressed in the established human ovarian surface epithelial (HOSE) cell line. (A) Schematic representation of the hSALL2 gene showing alternative first exons E1 (24 aa) and E1A (22aa) regulated by promoters P1 and P2, respectively, followed by the long common exon 2 (983 aa). Asterisks (***) indicate presence of a CpG island in P2. (B) Western blot of extracts from HOSE and P19 cells with antibodies to E1 and E1A. (C) RT‐PCR on RNA from HOSE cells with primers that bridge exon junctions.