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. 2013 Mar 21;7(3):719–731. doi: 10.1016/j.molonc.2013.03.001

Figure 6.

Figure 6

ST6GalNAc.I mRNA expression and sialyltransferase activity in bladder cancer MCR cell lines. A) ST6GalNAc.I expression and activity in MCR cell lines. The relative mRNA levels of ST6GalNAc.I (open bars) and sialyltransferase activity towards ABSM (grey bars) were analysed as described in the Material and methods section. Both, ST6GalNAc.I mRNA and sialyltransferase activity towards ABSM are negligible in negative control cells and markedly increased upon ST6GalNAc.I transduction. B) Flow cytometry analyses of transduced MCR cells. Both negative control (MCRnc in grey histogram) and ST6GalNAc.I‐transduced (MCRSTn+ in open histogram) cell lines were stained with the secondary antibody anti‐Ig's‐FITC following incubation with the primary antibody anti‐STn antigen. 90% of the ST6GalNAc.I‐transduced cells expressed the STn antigen (MFI = 216). The data are shown as a mean ± standard deviation of 3 independent studies. “*” p < 0.05, “**” p < 0.01 (Student's T‐test).