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. 2014 May 9;8(7):1306–1325. doi: 10.1016/j.molonc.2014.04.005

Figure 9.

Figure 9

Wnt‐Myc‐E2F mediated transcription program is activated in intestinal type gastric cancer cells. (A) Relative quantity of c‐Myc mRNA across selected low level G13 modular gene expressing (YCC16 and AGS) and high level G13 modular gene expressing (IM95 and YCC3) gastric cancer cell lines. This expression values are from the genome‐wide mRNA profile of GSE22183. (B) Immunoblot showing relative c‐Myc protein quantity in the same cell line panel. (C) Basal and un‐induced Wnt/TCF in‐vitro transcription factor reporter activity measured across selected gastric cancer cell lines. Reporter activity was measured by dual luciferase assay and expressed in folds of reporter activity in comparison to the firefly reporter without enhancer. (D) Confirmation of the repressed Wnt transcriptional activity upon silencing the CTNNB1 (β‐catenin) gene in AGS cells (Wnt hyperactive cell line) by transfecting the CTNNB1 siRNA. (E) RNAi mediated silencing of CTNNB1 results in the reduction in MYC & E2F reporter/transcriptional activity in AGS cells. (F) Model summarizing all the differential and contrasting features identified from this investigation for their association and involvement in intestinal and diffuse subtypes of gastric tumors. These are the novel molecular and cellular hallmarks of gastric cancer subtypes identified from the current investigation.