Figure 5.

Activated fibroblasts are SA‐β‐Gal and γ‐foci positive. A) HPFs, CAFs or in vitro activated CAFs were stained for SA‐β‐Gal. Counts of at least 200 cells were averaged and expressed as percent of positive SA‐β‐Gal staining ±SD. *p < 0.005 HPF + TGFβ or CAF vs HPF. B) Cell treated as in A) were fixed and examined by immunofluorescence for H2AX phosphorylated on Ser139 (γ‐H2AX) and counterstained with propidium iodide. Counts of at least 200 cells were averaged and expressed as percent of γ‐foci positive staining ±SD. *p < 0.005 HPF + TGFβ or CAF vs HPF.