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. 2014 Jan 24;8(3):642–655. doi: 10.1016/j.molonc.2014.01.007

Figure 3.

Figure 3

Transcriptional synergy depends on the distal STAT binding site in the FAT10 promoter. (A) Left, Diagram of the pFAT102.0kb‐luc construct, the 2.2 kb genomic DNA fragment of the FAT10, with predicted STAT and NFκB binding sites. Right, FAT10 promoter activation with pFAT102.0kb‐luc was measured after IL‐6 and/or TNF‐α stimulation (10 ng/ml each) for 6 h. (B, C) HepG2 cells were transfected with various reporter constructs, as indicated, and the synergistic FAT10 promoter activation was measured after IL‐6 and/or TNF‐α stimulation for 6 h. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student's t‐test.