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. 2014 Jan 2;8(3):469–482. doi: 10.1016/j.molonc.2013.12.014

Figure 4.

Figure 4

Preventive effect of AZD8055 on BMS‐777607‐induced senescence, polyploidy, and survivin expression in breast cancer cells. T‐47D and ZR‐75‐1 cells were cultured as described in Figure 3A and then treated for 72 h with BMS‐777607, AZD8055, and their different combinations. (A) Senescence was determined by detecting SABG and polyploidy was documented by staining nuclear DNA with DAPI. (B) The morphological appearance of polyploid cells also was determined by H&E staining and then photographed. (C) DNA contents were measured by the propidium iodide staining method followed by follow cytometric analysis. Arrows indicate 8N DNA contents. (D) Expression and localization of survivin and α‐tubulin were determined by immunofluorescent analysis using specific antibodies, followed by FITC or rhodamine‐labeled second antibodies as previously described (Sharma et al., 2013). Cells at metaphase were analyzed for expression and localization of survivin, α‐ tubulin, and their association with condensed DNA. Images were made at magnification of ×400. Data shown here are from one of three experiments with similar results.