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. 2014 Aug 27;9(1):249–259. doi: 10.1016/j.molonc.2014.08.002

Figure 2.

Figure 2

Aurora‐A stabilizes β‐catenin protein. A) The upregulated expression of β‐catenin protein in Aurora‐A overexpressing cells. β‐catenin, FAK, VHL, Caveolin‐1, E‐cadherin or Cyclin B1 protein and β‐catenin mRNA (Bottom) levels were examined using immunoblotting and RT‐PCR assay in control, Aur‐1 and Aur‐2 cells. B) The stabilization of β‐catenin in Aurora‐A overexpressing cells. The cells were treated with CHX and collected at the indicated times for immunoblotting analysis. C) The cells were treated with CHX in the presence of MG132, and subjected to immunoblotting analysis. D) The cell protein extracts from control, Aur‐1 and Aur‐2 cells were immunoprecipitated with β‐catenin antibody, followed by immunoblotting assay with ubiquitin antibody. E) The cell lysates were analyzed with antibodies to GSK‐3β, Axin and APC. F) The cellular protein were immunoprecipitated with anti‐Axin antibody, immunocomplexes were analyzed with antibodies against β‐catenin, APC and GSK‐3β.