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. 2014 Aug 27;9(1):249–259. doi: 10.1016/j.molonc.2014.08.002

Figure 5.

Figure 5

Aurora‐A phosphorylates β‐catenin at ser552 and ser675. The identification of phosphorylating regions of β‐catenin by in vitro Aurora‐A kinase assay. A series of purified GST‐β‐catenin, GST‐β‐catenin S552A, GST‐β‐catenin S675A or GST‐β‐catenin S552/675A proteins were used as substrates for purified Aurora‐A, and subjected to a kinase reaction with [γ‐32P]ATP. Whole kinase reactions were then subjected to electrophoresis followed by autoradiography (A and C). Sequence analysis of β‐catenin for candidate phosphorylation sites of Aurora‐A (B).