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. 2014 Aug 27;9(1):249–259. doi: 10.1016/j.molonc.2014.08.002

Figure 6.

Figure 6

β‐catenin phosphorylated at ser552 and ser675 by Aurora‐A increases its stability and transcriptional activity. A) GFP‐tagged WT β‐catenin, β‐catenin S552A, β‐catenin S675A or β‐catenin S552/675A vector was transiently expressed in Aur‐2 cells via Lipofectamine transfection. 48 h post‐transfection, the cells were treated with CHX and collected at the indicated times for immunoblotting analysis with anti‐GFP antibody. B) KYSE150 cells expressing GFP‐tagged WT β‐catenin, β‐catenin S552A, β‐catenin S675A or β‐catenin S552/675A were stained with DAPI. C) The luciferase reporter assay. TOP‐FLASH or FOP‐FLASH was co‐transfected with GFP‐tagged WT β‐catenin, β‐catenin S552A, S675A or S552A/S675A mutants in KYSE150 cells. Fold induction was calculated by normalizing luciferase activity of the cells expressing WT β‐catenin or mutant β‐catenin to that in the cells transfected by vector along (GFP). D) Analysis of Cyclin D1 and MMP7 mRNA levels in KYSE150 cells expressing GFP, GFP‐tagged β‐catenin, or β‐catenin S552/675A.