MiR‐449a was a tumor suppressor in human GBM cell lines and GSCs. (A) Relative expression of miR‐449a after cells transfected with pre‐miR‐449a and anti‐miR‐449a. (B) Effect of miR‐449a on the proliferation of U87, U251 and GSCs. (C) Effect of miR‐449a on the apoptosis of U87, U251 and GSCs. Effect of miR‐449a on cell migration (D) and invasion (E) of U87, U251 and GSCs. Data are presented as the mean ± SD (n = 5, each group). *P < 0.05 vs. pre‐NC group, #P < 0.05 vs. anti‐NC group. Scale bars represent 20 μm.