Table 1.

Obesity‐associated parameters in the experimental mice recorded at the end of the experiment. HFD C57BL/6J mice were divided into two major groups. One group was continuously fed with HFD, whereas the other group was shifted from HFD to normal diet. Mice of both the groups were orally treated with orlistat (10 mg/kg) or vehicle control on every alternate day for 8 weeks. Their body weight was monitored weekly throughout the study and serum was collected at the end of the experiment. Blood glucose, serum TG, serum cholesterol, serum LDLc and serum free fatty acids were measured. Serum factors including leptin, adiponectin, insulin, resistin, IL‐6 and TNF‐α were estimated by ELISA. Similarly, HFD NOD/SCID mice were treated orally with orlistat or vehicle control on every alternate day for 8 weeks and serum parameters were measured. Parallely, ND C57BL/6J mice were divided into two major groups. One group was orally treated with orlistat or vehicle control on every alternate day before inoculating B16F10 cells. The second group was orally treated with orlistat (10 mg/kg) or vehicle control on every alternate day after 2 days of inoculating B16F10 cells. The treatment was continued for 8 weeks. Their body weight was monitored weekly throughout the study and serum was collected at the end of the experiment for further biochemical analysis. The results are given as means ± standard deviation; *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant; #, comparison with C57BL/6J HFD‐HFD Ctrl; $, comparison with NOD/SCID HFD‐HFD Ctrl; §, comparison with C57BL/6J ND‐Pre Ctrl; ψ, comparison with C57BL/6J ND‐Post Ctrl.

Parameters	C57BL/6J HFD				NOD/SCID HFD		C57BL/6J ND
	HFD‐HFD Ctrl	HFD‐HFD Orli	HFD‐ND Ctrl	HFD‐ND Orli	HFD‐HFD Ctrl	HFD‐HFD Orli	ND‐Pre Ctrl	ND‐Pre Orli	ND‐Post Ctrl	ND‐Post Orli
Body weight (g)	32.35 ± 2.61	25.22#∗ ± 2.36	26.73#∗ ± 2.6	22.69#∗ ± 2.13	33.10 ± 2.18	27.22$∗ ± 2.19	20.61 ± 1.39	20.67§NS ± 1.71	20.81 ± 1.77	20.78 ψNS ± 1.67
Blood glucose (mg/dl)	192.34 ± 12.31	146.47#∗ ± 10.21	168.94#∗ ± 14.51	129.38#∗ ± 8.96	171.23 ± 22.33	143.45$∗ ± 15.52	162.24 ± 11.31	156.4§NS ± 10.21	161.63 ± 14.51	159.24ψNS ± 11.96
Serum TG (mg/dl)	84.54 ± 4.31	65.32#∗ ± 7.32	68.56#∗ ± 7.32	63.45#∗ ± 5.17	78.36 ± 5.62	66.35$∗ ± 5.37	56.54 ± 4.31	58.32§NS ± 5.61	55.36 ± 7.32	57.12ψNS ± 5.17
Serum cholesterol (mg/dl)	119.36 ± 8.96	84.36#∗ ± 6.98	97.54#∗ ± 7.39	83.5#∗ ± 5.43	107.56 ± 8.96	89.21$∗ ± 6.94	85.36 ± 8.96	84.43§NS ± 6.98	87.54 ± 7.39	86.50ψNS ± 5.43
Serum LDLc (mg/dl)	87.36 ± 6.46	69.23#∗ ± 5.37	72.94#∗ ± 6.38	67.96#∗ ± 4.31	89.37 ± 5.68	72.40$∗ ± 4.71	53.36 ± 6.46	54.23§NS ± 5.37	52.94 ± 6.38	52.96ψNS ± 4.31
Serum free fatty acids (mM/L)	2.56 ± 0.20	2.06#∗ ± 0.08	1.81#∗ ± 0.09	1.57#∗∗ ± 0.05	1.08 ± 0.05	0.66$∗ ± 0.06	0.89 ± 0.07	0.91§NS ± 0.06	0.90 ± 0.05	0.92ψNS ± 0.07
Serum leptin (ng/ml)	42.92 ± 4.93	29.55#∗ ± 2.45	35.5#∗ ± 1.69	24.1#∗∗ ± 1.37	36.70 ± 1.99	23.83$∗ ± 1.50	14.32 ± 1.93	13.98§NS ± 1.46	13.61 ± 1.70	14.38ψNS ± 1.38
Serum adiponectin (ng/ml)	3820 ± 432.97	8650#∗ ± 174.73	5805#∗ ± 238.42	9171#∗∗ ± 505.96	5426 ± 360.74	9726$∗∗ ± 310.05	12982 ± 832.97	12246§NS ± 674.74	13147 ± 738.23	13213ψNS ± 805.97
Serum insulin (μg/L)	0.3935 ± 0.03	0.2527#∗ ± 0.02	0.309#∗ ± 0.011	0.2401#∗ ± 0.014	0.3496 ± 0.027	0.225$∗∗ ± 0.011	0.1225 ± 0.03	0.114§NS ± 0.02	0.1162 ± 0.014	0.121ψNS ± 0.012
Serum resistin (ng/ml)	2.13 ± 0.13	1.51#∗ ± 0.16	1.66#∗ ± 0.14	1.1#∗∗∗ ± 0.09	1.13 ± 0.06	0.82$∗ ± 0.04	0.99 ± 0.09	1.01§NS ± 0.09	0.98 ± 0.10	0.99ψNS ± 0.08
Serum IL‐6 (pg/ml)	40.62 ± 4.13	29.62#∗ ± 2.90	33.45#∗ ± 2.86	26.8#∗∗∗ ± 3.72	33.17 ± 1.81	21.42$∗ ± 0.73	19.23 ± 2.13	20.32§NS ± 2.90	19.86 ± 2.87	19.45ψNS ± 2.73
Serum TNF‐α (pg/ml)	28.35 ± 3.01	14.02#∗ ± 1.42	17.6#∗ ± 1.51	12.07#∗ ± 1.73	24.06 ± 1.40	10.46$∗ ± 0.23	10.88 ± 2.01	11.05§NS ± 1.82	10.23 ± 1.52	11.12ψNS ± 1.73