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. 2015 Jun 5;9(9):1760–1772. doi: 10.1016/j.molonc.2015.05.007

Figure 2.

Figure 2

PEGylation does not result in major modifications of MvDN30 properties. (A) ELISA binding analysis of MvDN30, MvDN30‐PEG20 and MvDN30‐PEG40 (liquid phase) to a Met‐Fc chimera (solid phase). O.D.: optical density at 450 nm. Each point is the mean of triplicate values; bars represent standard deviation. (B) Met down‐regulation and shedding in A549 cells treated with increasing concentration of MvDN30, MvDN30‐PEG20 or MvDN30‐PEG40 for 48 h. Total Met levels (p145) and Met extracellular domains (p80) released in the extracellular environment were determined by Western blot analysis of cell extracts and of cell culture supernatants, using anti‐Met antibodies. The lysate filter was re‐probed with anti‐Vinculin antibodies. Bands were quantified and normalized against vinculin (p117); values in the top line boxes represent the percentage reduction of Met signal in the lysates extracted from treated samples with respect to the control and values in the bottom line boxes represent the fold increase of the Met signal in the treated samples with respect to the control, for the shown experiment. Data reported in the figure are representative of at least two experiments done.