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. 2015 Feb 9;9(6):1091–1105. doi: 10.1016/j.molonc.2015.01.007

Figure 3.

Figure 3

Ectopic TAZ overexpression induces EMT‐like changes A: TAZ overexpression was confirmed by western blot in cellular lysates from cells infected with TAZ cDNA lentivirus. Representative images are shown. B: Enforced TAZ overexpression resulted in EMT‐like morphological changes from cobble‐like to spindle‐like appearance under phase contrast microscopy. C, D: The cell motilities and invasion were remarkably enhanced after TAZ overexpression as gauged by wound healing assay(C) and transwell‐invasion assay(D). E: E‐cadherin expression was probed by immunoflurescent staining in TAZ‐overexpressing and control cells (left panel). Scale bar: 100 μm. The abundance of EMT markers E‐cadherin and vimentin following TAZ overexpression were assayed by western blot (right panel). Representative images are shown. F: The mRNA levels of EMT‐associated transcription factors and E‐cadherin/vimentin were determined by real‐time RT‐PCR. Data showed here are mean ± SD from three independent experiments, *p < 0.05, **p < 0.01, Student's t‐test.