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. 2014 Dec 29;9(4):791–805. doi: 10.1016/j.molonc.2014.12.007

Figure 5.

Figure 5

Paclitaxel‐resistant ovarian cancer cells show increased sensitivity independent of CAR‐mediated infection. A. SKOV3, SKOV3‐TR, A2780 and A2780tx1000 cells were infected with AdCMV‐GFP at MOI 10, 0.3, 10 and 1 respectively. The percentage of GFP positive cells was determined by flow cytometry. B. SKOV3, SKOV3‐TR, A2780 and A2780tx1000 cells were infected with dl922‐947 at MOI 10, 0.3, 10 and 1 respectively. Cell survival was determined 120 h later. ***; p < 0.001. C. Both cell pairs were infected with Ad11 and Ad35. Cell survival was determined 120 h later. D. SKOV3 and SKOV3‐TR cells were isoinfected with dl922‐947. Intracellular (above) and released (below) infectious virion production was quantified up to 72 h post‐infection by TCID50 assay. E. Protein was extracted from SKOV3 and SKOV3‐TR cells isoinfected with dl922‐947 up to 72 h post‐infection. Expression of E1A and adenoviral structural proteins was assessed by immunoblot.