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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 May 17;329:48–57. doi: 10.1016/j.taap.2017.05.019

Figure 2.

Figure 2

Effects of methyl- and butylparaben on osteoblastogenic differentiation of multipotent stem cell C3H10T1/2. C3H10T1/2 cells were induced for osteoblastogenic differentiation in the presence or absence of methyl- (100 μM), butylparaben (100 μM), or the vehicle control DMSO. (A) Runx 2 mRNA was analyzed 12 h (Left panel) and mRNA of ALP, OCA, and OPN was analyzed 12 days (Right panels) after the initiation of differentiation. Expression was normalized to 36B4 and expressed as fold of the controls (set at 1). (B) Microscopic images of the cells at day 12 were shown. Data are mean ± SEM (n=3). *,**, p<0.05 and p<0.01 versus the vehicle control DMSO, respectively.