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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 May 17;329:48–57. doi: 10.1016/j.taap.2017.05.019

Figure 5.

Figure 5

Adipogenic effects of butylparaben are attenuated by PPARγ knockdown, but not by GR knockdown in C3H10T1/2 cells. C3H10T1/2 cells with stable knockdown of PPARγ (PPARγ KD), GR (GR KD), or the scrambled controls (SCR) were seeded at the same density and induced for adipogenic differentiation for 8 days after reaching confluence in the presence or absence of methyl- (100 μM), butylparaben (100 μM) or the vehicle control DMSO. (A) mRNA expression of C/EBPα, FABP4, and PLIN were analyzed on day 8 after initiation of differentiation. Expression was normalized to 36B4 and expressed as fold of the controls (set at 1). (B) Quantifications of ORO absorbance at day 8 are shown. Data are mean ± SEM (n=3). *,**, p<0.05 and p<0.01 versus the SCR controls (A) or versus the vehicle control DMSO (B), respectively.