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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 May 17;329:48–57. doi: 10.1016/j.taap.2017.05.019

Figure 6.

Figure 6

Suppression of osteoblastogenic differentiation by paraben was affected by knockdown of either PPARγ or GR in C3H10T1/2 cells. C3H10T1/2 cells with stable knockdown of PPARγ (PPARγ KD), GR (GR KD), or the scrambled controls (SCR) were seeded at the same density (~ 5.0×10(4) per well of 12-well plates) and induced for osteoblastogenic differentiation for 12 days after reaching confluence. C3H10T1/2 cells were induced for osteoblastogenic differentiation in the presence or absence of methyl- (100 μM), butylparaben (100 μM), or the vehicle control DMSO. (A) mRNA expression of osteoblastogenic marker ALP, OCA and OPN were analyzed on day 12 after the initiation of differentiation. (B) mRNA expression of adipogenic and lipid associated marker C/EBPα, FABP4, and PLIN were analyzed on day 12 after the initiation of osteoblastogenic differentiation. Expression was normalized to 36B4 and expressed as fold of the controls (set at 1). Data are mean ± SEM (n=3). *,**, p<0.05 and p<0.01 versus the SCR controls, respectively.