Fig. 2. Tuft cells influence type 2 immunity through TRPM5.

(A) Gustducin, PLCβ2, and TRPM5 expression in sorted tuft cells, compared with the non-tuft cell epithelium. (B) Representative images of Tm-colonized WT and gustducin^−/− mice and tuft cell frequencies. (C) Representative image from Trpm5^eGFP mice. GFP is shown in green), DCLK1 in red), DAPI in blue, and phalloidin in white. (D) Representative image of Tm-colonized Trpm5^−/− mice and tuft cell frequencies. Scale bars in (B), (C), and (D), 50 μm. (E) Representative flow cytometry plots of IECs from uninfected (left) or Tm-colonized (right) WT (Gfi1b^eGFP/+, top) and Trpm5^−/− (Gfi1b^eGFP/+ Trpm5^−/−, bottom) mice and (F) tuft cell frequency. (G) Goblet cells in SI sections stained with Alcian blue and nuclear red in uninfected WT and Tm-colonized WT and Trpm5^−/− mice and (H) goblet cell frequency. (I) Eosinophil frequency in the distal SI lamina propria (LP) of uninfected and Tm-colonized WT and Trpm5^−/− mice. Scale bars, 50 μm. Each symbol represents an individual mouse, and all data are representative of at least three independent experiments. Data are plotted as means with SD. Four stars, P < 0.0001; three stars, P = 0.0001; two stars, P < 0.01; ns, not significant; one-way ANOVA, Kruskal-Wallis, or Mann-Whitney tests.