Skip to main content
. Author manuscript; available in PMC: 2017 Jul 26.
Published in final edited form as: Nat Neurosci. 2015 Jun 1;18(7):965–977. doi: 10.1038/nn.4030

Figure 2. Increased expression of maturation and activation marker in GF microglia.

Figure 2

(a) Gating strategy for flow cytometric analysis of CD11 b+ CD45lo microglia from GF and SFP mice. Representative dot plots obtained from three independent experiments are shown. SSC, side scatter. (b) Representative cytometry graphs of the maturation and activation marker CSF1R, F4/80, CD31, CD44, CD62L and MHC class II on microglia from GF mice (red lines), SFP mice (blue lines) and isotype controls (gray lines). In addition, quantifications of the percentages (%) of positively labeled cells and MFIs are depicted. Each symbol represents data from one mouse, with six investigated mice per group. Data are presented as mean ± s.e.m. Significant differences were determined by an unpaired t test (*P < 0.05, **P < 0.01, ***P < 0.001). Data are representative of three independent experiments. P values: CSF1R (percentage of positive cells), 0.0068; CSF1R (MFI), <0.0001; F4/80 (percentage of positive cells), 0.0134; F4/80 (MFI), 0.0047; CD31 (percentage of positive cells), 0.0103.