Association of CpG methylation of the TFAP2B locus with prognostic markers and TFAP2B expression in neuroblastoma. (a)
TFAP2B genomic structure and localization of CpGs analyzed. (b) Correlation of TFAP2B methylation with TFAP2B expression. Log intensities of probe A_24_P20954 (TFAP2B expression) are plotted against β‐values of probes cg21502834, cg06117072 and cg25593948 representing CpGs in the 5′ region of the TFAP2B locus. (c) Association of TFAP2B methylation (probe cg21502834) with MYCN amplification status, tumor stage, age at diagnosis, and risk group according to NB2004 in 105 neuroblastoma samples as determined by 450K methylation arrays. Boxes, median expression values (horizontal line) and 25th and 75th percentiles; whiskers, distances from the end of the box to the largest and smallest observed values that are <1.5 box lengths from either end of the box; circles, outliers. (*), Wilcoxen test (HR vs IR/LR); (***), p < 0.001; MNA, MYCN‐amplified tumors.