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. 2005 Feb 18;102(9):3447–3452. doi: 10.1073/pnas.0407759102

Fig. 2.

Fig. 2.

Transfection of CASMCs with Cy3-labeled double stranded-RNA. (A) Red fluorescence. (B) Green staining of nuclei proved CASMC viability 24 h after the transfection process by live/dead staining. (C) Transfection of CASMCs with siEgr duplexes but not with scRNA duplexes reduced Egr-1 mRNA expression by 60% 4 h after chlamydial infection (mean + SEM, n = 3 experiments).